Investigación

Líneas de Investigación

Desarrollo analítico aplicado a la biotecnología vegetal:

Espectroscopía molecular para la caracterización de recursos naturales vegetales (fenómenos de estrés, producción de biocombustibles, detección de patógenos, caracterización de biomasa lignocelulósica, estudios de micro y ultraestructura de tejidos vegetales).

Bioespectroscopía molecular:

Espectroscopía NIR, espectroscopía visible, espectroscopía de fluorescencia, espectroscopía infrarroja, técnicas de imágenes hiperespectrales, microscopía de fluorescencia confocal, imágenes químicas en el VIS-NIR.

Quimiometría y análisis multivariado de datos:

Clasificación y regresión multivariada de datos, análisis multivías, análisis de imágenes hiperespectrales.

Química de la madera y compuestos lignocelulósicos.

Control de calidad de productos y procesos farmacéuticos a través de técnicas alternativas de espectroscopía molecular. 

Publicaciones Científicas

  1. Camila Y. Bastidas, Rosario del P. Castillo, José Manuel Amigo, Carlos von Plessing, José Troncoso. Distributional homogeneity and penetration depth assessment of antibiotic added by surface coating to pellets with mid Infrared imaging and multivariate curve resolution. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2022 (271)- 120864. https://doi.org/10.1016/j.saa.2022.120864.
  2. Pino-Torres, César, Castillo-Felices, Rosario, Mundaca-Uribe, Rodolfo, Mendez-Herrera, Paola, Aranda-Bustos, Mario, & Peña-Farfal, Carlos. (2021). Development and assesment of spectroscopy methodologies and chemometrics strategies to detect pharmaceuticals blend endpoint in a pharmaceutical powder blend. Journal of the Chilean Chemical Society, 66(4), 5387-5397. https://dx.doi.org/10.4067/s0717-97072021000405387
  3. Mennickent, D., Castillo, R. d. P., Araya, J., Neira, J. Y. Analytical performance of Compton/Rayleigh signal ratio by total reflection X-ray fluorescence (TXRF): A potential methodological tool for sample differentiation. X-Ray Spectrom 2022, 51(2):142-150, https://doi.org/10.1002/xrs.3273
  4. Mario I. Sanhueza, Rosario del P. Castillo*, M.F. Meléndrez, Carlos von Plessing, JoannaTereszczuk, Germán Osorio, Carlos Peña-Farfal, Marcos Fernández, José Y.Neira. Confocal laser scanning microscopy as a novel tool of hyperspectral imaging for the localization and quantification of fluorescent active principles in pharmaceutical solid dosage forms. Microchemical Journal, 2021 (168), 106479. https://doi.org/10.1016/j.microc.2021.106479.
  5. Troncoso-Ortega, E.; Castillo, R.d.P.; Reyes-Contreras, P.; Castaño-Rivera, P.; Teixeira Mendonça, R.; Schiappacasse, N.; Parra, C. Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility. Biomolecules 2021, 11, 507. https://doi.org/10.3390/biom11040507
  6. Sanhueza, MI, Meléndrez, MF, von Plessing, C, Neira José, Y. Freer J, Castillo R del P*. Raman microimaging as an analytical technique for simultaneous quantification and localization of active principles in pharmaceutical solid dosage forms. J Raman Spectrosc. 2020; 51: 649– 659. https://doi.org/10.1002/jrs.5833
  7. César Pino-Torres, Santiago Maspoch, Rosario Castillo-Felices, Mónica Pérez-Rivera, Mario Aranda-Bustos, Carlos Peña-Farfal*. Evaluation of NIR and Raman spectroscopies for the quality analytical control of a solid pharmaceutical formulation with three active ingredients. Microchemical Journal, 2020, 154,104576. https://doi.org/10.1016/j.microc.2019.104576.
  8. Angelo Beratto-Ramos, Cristian Agurto-Muñoz, Juan Pablo Vargas-Montalba, Rosario del P. Castillo*. Fourier-transform infrared imaging and multivariate analysis for direct identification of principal polysaccharides in brown seaweeds. Carbohydrate Polymers, 2020, 115561, doi.org/10.1016/j.carbpol.2019.115561.
  9. Juan A. Araya*, Renato L. Carneiro, Juanita Freer, José Y. Neira, Rosario del P. Castillo. Fourier transform infrared imaging and quantitative analysis of pre-treated wood fibers: A comparison between partial least squares and multivariate curve resolution with alternating least squares methods in a case study. Chemometrics and Intelligent Laboratory Systems. 2019, 195: 103890, https://doi.org/10.1016/j.chemolab.2019.103890.
  10. Felipe Aravena-Sanhueza, Mónica Pérez-Rivera, Rosario Castillo-Felices, Rodolfo Mundaca-Uribe, Mario Aranda Bustos and Carlos Peña Farfal*. Determination of antioxidant capacity (ORAC) of Greigia sphacelata and correlation with voltammetric methods. 2020. Journal of the Chilean Chemical Society 65(3):4925-4928. http://dx.doi.org/10.4067/s0717-97072020000204925
  11. Ángela Álvarez, Jorge Yáñez*, Yamil Neira, Rosario Castillo-Felices, Patricio Hinrichsen. Simple distinction of grapevine (Vitis vinifera L.) genotypes by direct ATR-FTIR. Food Chemistry, 2020: 328, 127164. https://doi.org/10.1016/j.foodchem.2020.127164.
  12. Toro, C*.; Torres, S.; Parra, V.; Fuentes, R.; Castillo, R.; Díaz, W.; Reyes, G.; Balladares, E.; Parra, R. On the Detection of Spectral Emissions of Iron Oxides in Combustion Experiments of Pyrite Concentrates. Sensors 2020, 20, 1284. https://doi.org/10.3390/s20051284.
  13. Jonnathan Alvarez,Marizu Velasquez, Ashwin Kumar Myakalwar, Claudio Sandoval, Rodrigo Fuentes, Rosario Castillo, Daniel Sbarbaro and Jorge Yañez*. Determination of copper-based mineral species by laser induced breakdown spectroscopy and chemometric methods. Journal of Analytical Atomic Spectrometry, 2019(34): 2459-2468. DOI: 10.1039/c9ja00271e.
  14. Mendez, Paola, Christian Nuñez, Jaime  Cabrera-Pardo , Cristian Paz, Juan Manuel Barraza, Rosario Castillo and Carlos Peña-Farfal*. Adsorption ability of activated carbon obtained from sub-bituminous coal (lebu, chile) to capture trimethylamine. J. Chil. Chem. Soc. 2019, vol.64, n.3, pp.4582-4585. Issn 0717-9707.  Http://dx.doi.org/10.4067/s0717-97072019000304582.
  15. Valentina Manzo, Jairón Goya-Pacheco, Daniel Arismendi, Mercedes Becerra-Herrera, Alver Castillo-Aguirre, Rosario Castillo-Felices, Milton Rosero-Moreano, Eduardo Carasek, Pablo Richter*. Cork sheet as a sorptive phase to extract hormones from water by rotating-disk sorptive extraction (RDSE). Analytica Chimica Acta. 2019, 1087: 1-10. DOI: 10.1016/j.aca.2019.08.069
  16. Medina González, G*, Castillo, R d P, Neira, JY.  Multivariate calibration for the improvement of the quantification of cadmium in the presence of potassium as interferent by total reflection X‐ray fluorescence. X‐Ray Spectrometry. 2019; 48: 700– 707. https://doi.org/10.1002/xrs.3113
  17. Camila Y. Bastidas, Carlos von Plessing, José Troncoso, Mario I. Sanhueza, José Y. Neira, Rosario del P. Castillo*. Analytical strategies based on near infrared spectroscopy and multivariate calibration for rapid quantification of florfenicol at low-concentrations in medicated-feed pellets. Microchemical Journal, 2019, 147: 1068-1074. https://doi.org/10.1016/j.microc.2019.04.033
  18. Naysa Díaz; Mónica Pérez-Rivera; Rosario Castillo; Rodolfo Mundaca; Felipe Aravena; Mario Aranda; Carlos Peña-Farfal*. Effect of chemical and physical variables in the photo-electrochemical removal of estriol (E3) and 17--ethinylestradiol (EE2) in aqueous solution.Journal of the Chilean Chemical Society 2018, 63(4): 4250-4256. http://dx.doi.org/10.4067/S0717-97072018000404250 
  19. Angelo Alonzo Beratto Ramos, Rosario del Pilar Castillo, Nicolás A Troncoso, Andrés Agurto, Cristian Agurto*. Selection Criteria for high-value biomass: Seasonal and Morphological Variation of Polyphenolic Content and Antioxidant Capacity in Two Brown Macroalgae. Journal of Applied Phycology. (2018). https://doi.org/10.1007/s10811-018-1528-9.
  20. Mellado C, Figueroa T, Báez R, Castillo R, Melendrez M, Schulz B, Fernández K*. Development of Graphene Oxide Composite Aerogel with Proanthocyanidins with Hemostatic Properties as a Delivery System. ACS Appl Mater Interfaces. 2018, 7;10(9):7717-7729. DOI: 10.1021/acsami.7b16084.
  21. Camila Y. Bastidas, Carlos von Plessing, José Troncoso, Rosario del P. Castillo*. Evaluation of the microscopic distribution of florfenicol in feed pellets for salmon by Fourier Transform infrared imaging and multivariate analysis. Journal of Pharmaceutical and Biomedical Analysis 152 (2018) 257–263. doi: 10.1016/j.jpba.2018.02.002.
  22. Noemí Cruz, Cecillia A. Bustos*, María Graciela Aguayo, Alain Cloutier, Rosario Castillo. Impact of the Chemical Composition of Pinus radiata Wood on its Physical and Mechanical Properties Following Thermo-Hygromechanical Densification.  BioResources 13(2), 2018. DOI: 10.15376/biores.13.2.2268-2282.
  23. Angelo Beratto, Cristian Agurto, Juanita Freer, Nicolás Troncoso, Andrés Agurto, Carlos Peña-Farfal and Rosario del P. Castillo*.  Chemical characterization and determination of the antioxidant capacity of two brown algae with respect to sampling season and morphological structures using infrared spectroscopy and multivariate analyses. Applied Spectroscopy. 2017, 71(10):2263-2277. DOI: 10.1177/0003702817715654
  24. Juan A. Araya*, Renato L. Carneiro, Cristian Arévalo, Juanita Freer, Rosario del P. Castillo. Single pixel quantification strategies using middle infrared hyperspectral imaging of lignocellulosic fibers and MCR-ALS analysis.  Microchemical Journal, 2017, 134:164-172. https://doi.org/10.1016/j.microc.2017.05.019.
  25. Cristian Arévalo; Juanita Freer; Pamela A. Naulin; Nelson Barrera; Eduardo Troncoso; Juan Araya; Carlos Peña-Farfal; Rosario del P. Castillo*.  Study of the ultrastructure of Eucalyptus globulus wood substrates subjected to auto-hydrolysis and diluted acid hydrolysis pre-treatments and its influence on enzymatic hydrolysis. Bioenergy Research (2017), 10(3): 714-727. DOI:10.1007/s12155-017-9833-8.
  26. Eduardo Troncoso, Rosario Castillo, Roberto Valenzuela, Pablo Reyes, Juanita Freer, Marcela Norambuena, Jaime Rodriguez, Carolina Parra*. Chemical And Microstructural Changes In Eucalyptus globulus Fibers Subjected To Four Different Pretreatments And Their Influence On The Enzymatic Hydrolysis. Journal of Chilean Chemical Society (2017). 62(2): 3442-3446. http://dx.doi.org/10.4067/S0717-97072017000200005.
  27. Soraya Andrea Bravo Muñoz*, Ariana Bertín Aileen Turner Francisco Sepúlveda, Paz Jopia, María José Parra, Rosario Castillo and Rodrigo Hasbún. Differences in DNA methylation, DNA structure and embryogenesis-related gene expression between embryogenic and non embryogenic lines of Pinus radiata D. don.  Plant Cell, Tissue and Organ Culture, 2017, 130:521–529. https://doi.org/10.1007/s11240-017-1242-3
  28. R. Mundaca-Uribe; D. Valencia-Muñoz; R.  Castillo Felices; J. N. Hinojosa; M. A. Bustos; C. Peña-Farfal*.Bienzymatic Biosensor for Malic Acid Based on Malate Dehydrogenase and Transaminase Immobilized onto a Glassy Carbon Powder/Carbon Nanotubes/Nad+ Composite Electrode. Electroanalysis 2017 (29):238-243. https://doi.org/10.1002/elan.201600295.
  29. Soraya Bravo*; María José Parra; Rosario Castillo; Francisco Sepúlveda; Aileen Turner; Ariana Bertín; Germán Osorio; Joanna Tereszczuk; Carola Bruna; Rodrigo Hasbún. Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav. Gayana Botánica, 2016. 73( 2): 402-413. http://dx.doi.org/10.4067/S0717-66432016000200402.
  30. Paola Méndez A, Camila Rivera A , Cesar Pino A , Rodolfo Mundaca B , Rosario Castillo B , José Neira B , Carlos Peña-Farfal. Chemical Characterization Of Sub-Bituminous Coal From The Arauco Province – Chile.  J. Chil. Chem. Soc.(2016), 61(1): 2805-2808. http://dx.doi.org/10.4067/S0717-97072016000100012.
  31. Mónica  La Torre, Rodrigo Vidal, Elizabeth González , Rosario Castillo, Carlos Peña-Farfal, Leonardo Bennunc and José Y. Neira*. Analytical testing of the interference standard method (IFS) for metals in wines by inductively coupled plasma mass spectrometry. J. Chil. Chem. Soc., 2015, 60(3): 3083-3087. http://dx.doi.org/10.4067/S0717-97072015000300023.
  32. Rosario del P. Castillo*, Juan Araya, Eduardo Troncoso, Silenne Vinet, Juanita Freer. Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process. Analytica Chimica Acta, 2015, 866: 10-20 https://doi.org/10.1016/j.aca.2015.01.032
  33. Rosario del P. Castillo*, Carolina Parra, Eduardo Troncoso, Heriberto Franco, Samuel Peña, And Juanita Freer. NIR Spectroscopy applied to the characterization and selection of pre-treated materials from multiple lignocellulosic resources for bioethanol production. Journal of Chilean Chemical Society, 2014, 59(1): 2347-2352. http://dx.doi.org/10.4067/S0717-97072014000100022.
  34. Rosario del P. Castillo*, Jaime Baeza, Joselyn Rubilar, Álvaro Rivera, Juanita Freer. Infrared Spectroscopy as Alternative to Wet Chemical Analysis to Characterize Eucalyptus globulus Pulps and Predict Their Ethanol Yield for a Simultaneous Saccharification and Fermentation Process.  Applied Biochemistry and Biotechnology. 2012,  168: 43-48. https://doi.org/10.1007/s12010-012-9915-1
  35. Jorge Yañez*, María Paz Farías, Valeria Zúñiga, César Soto, David Contreras, Eduardo Pereira, Héctor D. Mansilla, Rosario Castillo, Pedro Sáez. Differentiation of two main ammunition brands in Chile by Regularized Discriminant Analysis (RDA) of metals in gunshot residues. Microchemical Journal, 2012, 101:43-48. https://doi.org/10.1016/j.microc.2011.10.003
  36. María Graciela Aguayo, Licarayen Quintupil, Rosario Castillo, Jaime Baeza, Juanita Freer, Regis T. Mendonca. Determination of differences in anatomical and chemical characteristics of tension and opposite wood of 8-year old Eucalyptus globulus. Maderas. Ciencia y Tecnología 12(3), 241-251 (2010). http://dx.doi.org/10.4067/S0718-221X2010000300008.
  37. Rosario del P. Castillo*, David Contreras, Jaime Baeza, Cristian Agurto and Juanita Freer. Classification of genotypes of Eucalyptus globulus under cold conditions using their free amino acids content on leaves and regularized discriminant analysis (RDA). Journal of the Chilean Chemical Society  (2010), 55(1), 11-18  http://dx.doi.org/10.4067/S0717-97072010000100005
  38. Castillo, R. del P*, Contreras, D., Otto, M., Baeza, J. y J. Freer. Foliar damage assessment and selection of cold resistant genotypes using near infrared spectra of Eucalyptus globulus Leaves. Journal of Near Infrared Spectroscopy (2009), 17, 223-231 https://doi.org/10.1255/jnirs.845
  39. Rosario Castillo, David Contreras*, Juanita Freer*, José Ruiz, Sofía Valenzuela. 2008. Supervised pattern recognition techniques for classification of Eucalyptus species from leaves NIR spectra.  Journal of the Chilean Chemical Society  53(4), 1709-1713 (2008). http://dx.doi.org/10.4067/S0717-97072008000400016.
  40. Rosario Castillo*, Matthias Otto, Juanita Freer, Sofía Valenzuela. Multivariate strategies for classification of Eucalyptus globulus genotypes using carbohydrates content and NIR spectra for evaluation of their cold resistance. Journal of Chemometrics 2008, 22(3-4): 268-280,.  https://doi.org/10.1002/cem.1126
  41. J. Guillermo Contreras*, Márcia M.C. Ferreira, Marcela Hurtado, Lorena Gerli, Rosario Castillo. Theoretical and chemometric study of substituted oxazines. Journal of the Chilean Chemical Society 2005, 3, 50-54 http://dx.doi.org/10.4067/S0717-97072005000400015.

Proyectos de Investigación

Project 101008228

SuChAQuality. Sugar & Chocolate, Authenticity and Quality. Marie Skłodowska-Curie Research and Innovation Staff Exchange MSCA- RISE. Programa Horizon 2020. European Comission.  2021-2024. Institución Principal:  Middle East Technical University, Turkey. Investigador Responsable: Dr. Mecit Oztop. Investigadora Responsable en Chile: Dra. Rosario del P. Castillo.

www.suchaquality.com

Mas info: SuChAQuality aims to form an international and cross-sectoral network of organizations having expertise and skills in different areas to develop novel analytical methodologies to tackle the authenticity and quality problems of sugar and confectionery industries. SuChAQuality will enhance the quality of R&I in Europe, promoting cooperation along the ‘Sugar’ Value Chain actors. This is achieved through advanced international, intersectoral, cooperation between 7 academic and 5 industry partners who have in-depth knowledge of food science, chemistry, physics, design, production, biotechnology, statistics, mathematics and engineering. The participants of the network exchange skills and knowledge which will allow them to progress towards making key advances in new food analysis techniques, while strengthening the collaborative research between different countries and sectors. Advances in quality and authenticity methods that will be developed within the scope of the project bring affordable and convenient solutions for non-academic participants which will help to protect the quality and authenticity of their products thus reducing their waste. In turn, this will bring both cost savings to the consumers of such products and ensure that society as a whole benefit from a stable supply of authentic and good quality products. The staff members who participate in the project develop new skills, be exposed to new research environments and have their career perspectives widened.

Fondecyt Regular 1191281

Evaluation of the performance of confocal laser-scanning fluorescence microscopy (CLSFM) enhanced by advanced chemometric methods for direct solid analysis of pharmaceutical forms and samples with high topochemistry functionality dependence (2019-2022). ANID – Chile.  Investigadora Responsable. Dra. Rosario Castillo. Coinvestigadores: Marcos Fernández, Yamil Neira, Manuel Meléndrez, Carlos von Plessing.

Mas info: This project proposes the evaluation of the performance of the confocal laser scanning fluorescence microscopy (CLSFM) as hyperspectral imaging technique, for analysis of SDPF and its comparison with emerging analytical techniques based on hyperspectral imaging such as FT-IR imaging, NIR chemical imaging and Raman imaging for the identification and localization of fluorescent chemical components in solid dosage pharmaceutical forms (SDPF). The project attempts obtain the advantages of a 3D reconstruction, higher sensibility and simple experimental images acquisition regarding the existent technologies. Besides, characteristics as homogeneity of the active ingredients distribution, presence of contaminants, quantification of active principles, dissolution, uniformity of content and drug delivery of SDPF, will be evaluated using the developed analytical technique, in order to obtain a complete qualitative and quantitative quality control of SDPF, useful for drugs identification, quantifications, adulterations and counterfeits evaluations, with a modern conjugation of spectroscopy and microscopy.

Otros Proyectos

– Servicio de Caracterización Químico-Espacial y Cuantificación de Componentes de Comprimidos Farmacéuticos a través de nuevos métodos basados en Espectroscopía Vibracional.
VIU19P0074 FONDEF VIU (2019). Investigadora responsable: Camila Bastidas H., Profesor Guía: Dra. Rosario Castillo F.

– “Desarrollo y aplicación de una herramienta de detección temprana de oídio en VID y su prospección en la Viña de Neira para la reducción de pérdidas por procesos de infección”. Voucher de innovación para empresas de mujeres 2018 – 18VIPM -92600 CORFO (Agosto 2018-Marzo 2019). Directora del Proyecto: Dra. Rosario Castillo Felices. Co-investigadores: Dr. Yamil Neira, Dr. Juan Araya.

– Proyecto Enlace VRID-UDEC 217.071.065-1.0 “New analytical approaches based on hyperspectral imaging for evaluation of chemical and physical characteristics of solid dosage pharmaceutical samples: enhanced confocal laser scanning microscopy (CLSM) by application of multivariate image analysis” (Abril 2017- Marzo2019).

Investigadora Responsable: Dra. Rosario Castillo Felices.

– APY-GC- 2016-39 “Capacitación en herramientas Quimiométricas para la Investigación”. Secretaría Nacional de Ciencia, Tecnología e Innovación, Gobierno de la República de Panamá. Concurso adjudicado para capacitación en Quimiometría al personal del Centro de Investigación en Bioquímica y Química Aplicada (CIBQUIA) de la Universidad Autónoma de Chiriquí (UNACHI), Chiriquí, David, Panamá (Junio 2016 – Marzo 2017). Investigador Responsable Panamá: Dra. Mariel Monrroy. Investigador Visitante: Dra. Rosario Castillo Felices.

– ECIP-1-P-050-14 “Quimiometría y Espectroscopía Aplicada para la investigación y desarrollo de Productos Naturales de la Biodiversidad Peruana”. Programa Nacional de Innovación para la Competitividad y Productividad – PNICP de la República del Perú. (Febrero 2015 – Noviembre 2015). Contrato Nº 033-PNICP-ECIP-2015.

Investigador responsable Perú: Dra. Rosario Rojas. Investigadora contraparte extranjera: Dra. Rosario Castillo F.

– FONDECYT Iniciación 11130388: “Combined effects of chemical and physical characteristics of lignocellulosic biomass: Advanced analytical tools for a micro and nanoscale study of recalcitrance”. CONICYT – Chile. (2013- 2015). 

Investigadora responsable: Dra. Rosario Castillo Felices.

– FONDECYT POSTDOCTORAL 3100078: FT-NIR spectroscopy for a fast classification and compositional analysis of P. radiata and E. globulus wood samples in the bioethanol production process. CONICYT – Chile. (2009-2012).

Investigadora responsable: Dra. Rosario Castillo F.

– PFB27 – PCS011 (Proyecto Capital Semilla, Fondos de Financiamiento Basal): “Uso de la espectroscopía FT-IR para la caracterización, cuantificación y estudio de las transformaciones químicas de componentes lignocelulósicos en procesos que incrementen su valor agregado”. Unidad de Desarrollo Tecnológico (UDT), Universidad de Concepción. 2012.

Investigadora Responsable: Dra. Rosario Castillo.

– Fondecyt Regular 1201338. Improvement of The Interfactal Strength (IS) Of A Unidirectional Nano-engineered Composite Material Reinforced With Functionalized Nanostructures Grown Vertically On Recycled Carbon Fibers. Coinvestigador. 2020-2023.

Investigador Responsable. Dr. Manuel Meléndrez Castro. Coinvestigadores: Dra. Katherina Fernández, Dra. Rosario Castillo Felices.

– “Centro de Microscopía avanzada CMA – Bío Bío” Programa de Investigación Asociativa (PIA-CONICYT).

Miembro del Comité Científico: Dra. Rosario Castillo Felices. (2012 – 2020).

– Contrato Tecnológico  CORFO 16PIDE-66749 «Análisis químico en línea de materias primas en hornos de fusión bath smelting mediante espectroscopia laser». Contrato Tecnológico. 2017-2018.

Investigador responsable: Dr. Carlos Saavedra R., Dr. Jorge Yañez Solorza. Investigadora asociada: Dra. Rosario Castillo F.